OpenCRISPR-1 is an AI-designed, RNA-programmable gene editing system, offering a novel Cas9-like protein with an NGG PAM preference. It's intended for researchers and developers in the gene editing field, providing a drop-in replacement for existing Cas9 systems and enabling advanced techniques like base, prime, and epigenome editing.

How It Works

OpenCRISPR-1 is a Cas9-like protein engineered entirely by Profluent's large language models. While retaining the core architecture of Type II Cas9 nucleases, it features hundreds of mutations distinguishing it from natural variants. This AI-driven design approach allows for the creation of highly functional and potentially novel gene editing tools.

Quick Start & Requirements

• Access to the protein sequence and compatible guide RNA is available via a preprint.
• A license agreement is required for commercial use, which includes obligations for ethical use.
• General protocols for editing enzymes should be compatible.

Highlighted Details

• AI-generated, RNA-programmable gene editor with NGG PAM preference.
• Can be used as a drop-in replacement for SpCas9 in many protocols.
• Compatible with canonical SpCas9 guide RNAs.
• Can be fused in deactivated or nickase formats for advanced gene editing techniques.

Maintenance & Community

• The project is released by Profluent Bio.
• Users are encouraged to provide feedback and share their experiences.
• Profluent Bio offers collaborative customization for specific therapeutic applications.

Licensing & Compatibility

• OpenCRISPR is free for research and commercial usage.
• A license agreement is required for commercial use, stipulating ethical use obligations.
• The sequence is available via a preprint.

Limitations & Caveats

• While the sequence is freely available, a license agreement is necessary for commercial applications, which may introduce administrative overhead.
• Users are asked to acknowledge Profluent Bio as a user and notify them of clinical or commercial advancements.